Description
Description: This assay employs a two-site sandwich ELISA to quantitate KCNS3 in samples. An antibody specific for KCNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KCNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Voltage-gated potassium channels form the largest and most diversified class of ion channels and are present in both excitable and nonexcitable cells. Their main functions are associated with the regulation of the resting membrane potential and the control of the shape and frequency of action potentials. The alpha subunits are of 2 types: those that are functional by themselves and those that are electrically silent but capable of modulating the activity of specific functional alpha subunits. KCNS3 is not functional by itself but can form heteromultimers with member 1 and with member 2 (and possibly other members) of the Shab-related subfamily of potassium voltage-gated channel proteins. This gene belongs to the S subfamily of the potassium channel family